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primary antibodies against p-h2a.x  (GeneTex)

 
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    GeneTex primary antibodies against p-h2a.x
    Primary Antibodies Against P H2a.X, supplied by GeneTex, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primary+antibodies+against+p-h2a%2Ex/primary+antibodies+against+p+h2a+x/pmc10363793-41-14-12
    Average 90 stars, based on 1 article reviews
    primary antibodies against p-h2a.x - by Bioz Stars, 2026-09
    90/100 stars

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    other:

    Article Title: Patchouli alcohol induces G 0 / G 1 cell cycle arrest and apoptosis in vincristine‐resistant non‐small cell lung cancer through ROS ‐mediated DNA damage
    Article Snippet: The specific primary antibodies used in this study included those against p‐H2A.X (GeneTex, USA), p‐CHK1 and p‐p53, p‐CHK2 (Biorbyt Ltd.), p21, CDK2, cyclin E1, ERK, p‐ERK, AKT, p‐AKT, Bcl‐2, Bax, caspase‐9, caspase‐3, CD133, CD44, EpCAM, β‐actin (iReal Biotechnology), and P‐gp (Bioss Antibodies Inc.).

    Article Title: Patchouli alcohol induces G 0 /G 1 cell cycle arrest and apoptosis in vincristine-resistant non-small cell lung cancer through ROS-mediated DNA damage.
    Article Snippet: Patchouli alcohol (purity ≥98%) and vincristine (purity ≥98%) were purchased from the Wuhan ChemFaces Biochemical Co., Ltd, and cisplatin (purity ≥95%) was purchased from Cayman Chemical Company.



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    Ionizing radiation induces DNA damage. (A) Timeline of the experimental protocol performed to investigate epithelial cell unjamming induced by ionizing radiation. In primary HBE cells maintained in air-liquid interface culture exposed to ionizing radiation (IR), we determined DNA damage, barrier integrity, cellular viability, epithelial differentiation, as well as cellular shape and migration. (B) Representative images of p-H2AX (top, red) and nuclei stained with Hoechst (bottom, blue) from six independent experiments. IR exposure induced p-H2AX indicating increased DNA damage. Images were captured with a 63X objective (scale bars = 20 μm). The quantification of the mean area of positive p-H2AX staining is presented in the graph. Error bars represent the standard deviation from four FOVs ( n = 4) from one representative experiment. (C) Representative orthogonal images of p-H2AX (red), F-actin (green), and nuclei stained with Hoechst (blue). Images were captured with a 63X objective (scale bar = 20 μm). (D) Western blotting confirms that IR induced p-H2AX. Quantified p-H2AX normalized to E-cadherin is presented in the graph. Error bars represent the standard deviation from two samples ( n = 2).

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: Irradiation Induces Epithelial Cell Unjamming

    doi: 10.3389/fcell.2020.00021

    Figure Lengend Snippet: Ionizing radiation induces DNA damage. (A) Timeline of the experimental protocol performed to investigate epithelial cell unjamming induced by ionizing radiation. In primary HBE cells maintained in air-liquid interface culture exposed to ionizing radiation (IR), we determined DNA damage, barrier integrity, cellular viability, epithelial differentiation, as well as cellular shape and migration. (B) Representative images of p-H2AX (top, red) and nuclei stained with Hoechst (bottom, blue) from six independent experiments. IR exposure induced p-H2AX indicating increased DNA damage. Images were captured with a 63X objective (scale bars = 20 μm). The quantification of the mean area of positive p-H2AX staining is presented in the graph. Error bars represent the standard deviation from four FOVs ( n = 4) from one representative experiment. (C) Representative orthogonal images of p-H2AX (red), F-actin (green), and nuclei stained with Hoechst (blue). Images were captured with a 63X objective (scale bar = 20 μm). (D) Western blotting confirms that IR induced p-H2AX. Quantified p-H2AX normalized to E-cadherin is presented in the graph. Error bars represent the standard deviation from two samples ( n = 2).

    Article Snippet: Cells were then permeabilized in 0.2% Triton X-100 in phosphate-buffered saline (PBST) for 10 min. Permeabilized cells were blocked in 10% non-specific goat serum and 1% bovine serum albumin (BSA) in PBST for 1 h. Primary antibody against p-H2AX (20E3, Cell Signaling Technology, Danvers, MA) was diluted at 1:200.

    Techniques: Migration, Staining, Standard Deviation, Western Blot